发明名称 |
NOVEL CYCLODEXTRIN GLUCANOTRANSFERASE, PROCESS FOR PRODUCING THE SAME AND PROCESS FOR PRODUCING CYCLODEXTRIN BY USING THIS ENZYME |
摘要 |
CGTase having the following enzymatic chemical properties: (1) Function and substrate specificity: funtioning on starch, dextrin, amylopectin, and amylose to produce primarily gamma -CD, with the quantities of beta - and alpha -CD produced being smaller than the quantity of gamma -CD produced; (2) Optimum pH: 10.5-11.0; (3) Optimum temperature: About 60 DEG C; (4) Stable pH: 6-11; and (5) Temperature stability: With a 15 minute-treatment at 50 DEG C, residual activity of not less than 90 percent is exhibited. A method of manufacturing cyclodextrin glucanotransferase in which a microorganism belonging to the species Bacillus clarkii (for example, FERM BP-7156) and having the ability to produce cyclodextrin glucanotransferase is cultured, cyclodextrin glucanotransferase is produced in the cultured product, and the cyclodextrin glucanotransferase that has been produced is collected. A method of manufacturing CD in which CGTase produced by the species Bacillus clarkii is reacted with a solution comprising starch or the like to produce principally gamma -cyclodextrin. gamma -Cyclodextrin glucanotransferase (CGTase) capable of predominantly producing gamma -CD, a microorganism having the ability to produce gamma -CGTase, and a method of manufacturing gamma -CD using gamma -CGTase are provided. |
申请公布号 |
EP1308508(B1) |
申请公布日期 |
2008.08.20 |
申请号 |
EP20010932221 |
申请日期 |
2001.05.23 |
申请人 |
NIHON SHOKUHIN KAKO CO., LTD. |
发明人 |
TAKADA, MASAYASU;IDE, TAKAHIRO;YAMAMOTO, TAKESHI;UNNO, TAKEHIRO;WATANABE, YOSHIMI;SONE, HIRONOBU;YAMAMOTO, MIKIO |
分类号 |
C12N9/10;C12N1/20;C12P19/18;C12R1/07 |
主分类号 |
C12N9/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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