METHOD FOR MICROCHANNEL SURFACE MODIFICATION

摘要

A method (10) for functionalizing microfluidic channels with specific characteristics by the introduction of hydroxyl groups onto the surface area of the channel using a solution of an acid and a peroxide (30) followed by a reaction of an essentially pure siloxane-based reagent (60) and a funtional group (70). Chemically modified channel surfaces can be designed to prevent non-specific binding or interaction of biological samples with the channel surfaces; surface wetting or dewetting with the control of the hydrophobicity and hydrophilicity of the channel surfaces; confer solvent resistance by attaching inert molecules to the interior channel; and tethering molecules that have available amine, thiol, isocyanato and isothiocyanato or similar groups to permit the attachment of biomolecules to channel surfaces.