METHODS OF INHIBITING ANGIOGENESIS AND TUMOR DEVELOPMENT

摘要

The invention provides methods for identifying genes and proteins modulated by an antagonist. The antagonist can be to a cryptic epitope of an ECM component that specifically binds to the ECM component or an antagonist of avß3 that inhibits binding of avß3 to an ECM component. It additionally provides methods for using the products of the identified genes, or for using the identified proteins, for inhibiting angiogenesis, tumor metastasis, and other tumor developmental processes, including cell migration, cell adhesion, cell proliferation, and tumor growth and for treating angiogenesis-dependent conditions. The present invention relates to antagonists of cryptic epitopes of ECM components, wherein binding of these antagonists to cryptic epitopes of ECM components results in modulation of the expression of IGFBP-4, TSP-I, Id-I, p27^KIP or p21^CIP. The present invention relates to antagonists of avß3, wherein binding of these antagonists to avß3 results in modulation of the expression of IGFBP- 4 or TSP-I. The present invention also relates to methods of using these antagonists for inhibiting angiogenesis, tumor metastasis, and other tumor development processes as well as for treating angiogenesis-dependent conditions. The invention provides compositions comprising IGFBP-4 and methods for inhibiting angiogenesis and tumor development processes, and for treating angiogenesis-dependent conditions, using an insulin growth factor binding protein, IGFBP-4.