摘要 |
<p><P>PROBLEM TO BE SOLVED: To provide a method for collecting the cells that are expected to have been deeply participating to the carcinogenesis among the long-term survival cells. <P>SOLUTION: The method for collecting aged epithelial stem shells comprises the following (1) through (3) steps: the step (1) where a suspension of epithelial cells is added dropwise to a culture vessel covered with an extracellular adhesion whereby epithelial cells are adhered to the culture vessel; the step (2) where the culture medium is added to the culture vessel to which epithelial cells are adhered and the epithelial cells are cultured; and the step (3) where the survival cells are collected after the differentiated keratinocyte growing during the cultivation are exterminated. <P>COPYRIGHT: (C)2004,JPO&NCIPI</p> |