Method for producing recombined polynucleotides

摘要

The present invention concerns briefly a method for producing recombined polynucleotides by utilizing nucleotides or nucleotide analogues not normally present in naturally occurring polynucleotides, wherein the sugar-base bonds are cleavable, or from which the base-moiety can be cleaved, thus generating so-called AP-sites. These AP-sites may be used for generating random sized polynucleotide fragments for use in a shuffling procedure.