发明名称 |
Methods for determining single nucleotide variations |
摘要 |
The present invention provides a variety of methods for determining the identity of a nucleotide present at a variant site in a target nucleic acid. The methods involve conducting partial chain termination sequencing reactions of a target nucleic acid of interest conducted with the four deoxynucleotides but only one to three non-extendible nucleotides (i.e., analogs of dATP, dTTP, dGTP and dCTP that are non-extendible by a polymerase). The non-extendible nucleotide(s) utilized in the sequencing reactions are selected to be complementary to the bases potentially occupying the variant site of a target nucleic acid that serves as a template during sequencing. Analyses can also be performed in multiplex formats.
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申请公布号 |
US2001049102(A1) |
申请公布日期 |
2001.12.06 |
申请号 |
US20010792413 |
申请日期 |
2001.02.23 |
申请人 |
HUANG XIAOHUA C.;GILCHRIST MICHAEL JAMES;BENTLEY L. GORDON;RIENHOFF HUGH Y. |
发明人 |
HUANG XIAOHUA C.;GILCHRIST MICHAEL JAMES;BENTLEY L. GORDON;RIENHOFF HUGH Y. |
分类号 |
C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C12Q1/68 |
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