| 摘要 |
The present invention provides novel nucleic acid detection methods based on nucleic acid template-directed primer extension assays. Also provided are kits and genetic analysis methods. Included are microsequencing methods comprising detecting an incorporated nucleotide by measuring direct fluorescence intensity, fluorescence-based nucleic acid detection methods using oligonucleotide primers hybridizing to both complementary strands of a nucleic acid sample, and methods for decreasing background signal in nucleic acid template-directed primer extension assays. |
