发明名称 |
Process for the production of proteins in soluble form by modulation of an inducible promoter |
摘要 |
The present invention provides a process for the expression in appropriate host cells of recombinant DNA which is under the control of an inducible promotor. In order to obtain the soluble form of recombinant protein, which would usually be obtained in insoluble form, the induction of the promotor is limited to less than 10% of the maximum induction in comparison with a standard system and the transcription rate of the DNA coding for the protein is correspondingly limited.
|
申请公布号 |
US6284484(B1) |
申请公布日期 |
2001.09.04 |
申请号 |
US19910725943 |
申请日期 |
1991.06.27 |
申请人 |
ROCHE DIAGNOSTICS GMBH |
发明人 |
KOPETZKI ERHARD;SCHUMACHER GüNTHER |
分类号 |
C12N15/09;C12N1/38;C12N15/67;C12N15/70;C12P21/00;C12P21/02;C12R1/19;(IPC1-7):C12P21/02 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|