METHODS FOR IMPROVING VIRAL VECTOR TITERS AND REDUCING CELL DEATH IN CELL CULTURES

摘要

Disclosed are compositions and methods for the increasing the production of a recombinant viral vector in a cell culture, comprising culturing a recombina nt viral vector producing cell line in a basal media which further comprises (a ) greater than 1.5 g/L amino acids (or, a selected amino acids in a quantity a nd amount greater than present in a basal media such as DMEM), and (b) between 0.5% and 4% serum, such that the recombinant viral vectors are produced.