摘要 |
The invention relates to a process for preparing immunogenic conjugates, consisting of the quick addition of an aqueous solution comprising 1.15 parts of bovine serum albumin, at a temperature of 4 degree C, to 0.221 parts of an organo-chlorinated pesticide comprising an acid group and an aromatic ring for the activation of the carboxyl group, said mixture being left to rest for 20 hours, at a temperature of 4 degree C, and further centrifuged at 3,000 rpm for 30 min, the resulting precipitate being further dialysed four times, for 72 hours, with a solution 1 : 2 v/v of dioxan : water and vacuum dried, the parts being expressed by weight. The employed pesticides are: 2,4-dichlorophenoxy-acetic acid and 2-methoxy-3,6-dichlorobenzoic acid.
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