Ribozymal nucleic acids cleaving ccr5 or cxcr4

摘要

A vector system which can be incorporated in liposomes, comprising at least one DNA vector, the vector or vectors containing a target-cleaving hammerhead ribozymal DNA sequence under control of a promoter effective in human cells and which, upon transcription to RNA will cleave the mRNA transcribed from a target gene encoding the CCR5 or CXCR4 protein. Preferably the ribozymal DNA contains a first recognition sequence (5' to 3'): tagattg or ctcact, respectively for CCR5 or CXCR4 and downstream thereof a second recognition sequence acttg or acgttgt respectively for CCR5 or CXCR4.