发明名称 |
METHOD AND APPARATUS FOR CRYOPREPARATION, DRY STABILIZATION AND REHYDRATION OF BIOLOGICAL SUSPENSIONS |
摘要 |
<p>This invention relates to methods, apparatus and solutions for cryopreserving microscopic biological materials for biologically extended periods of time. The method comprises treating a suspension of biological material, in a appropriate buffer, with a cryoprotectant or combination of cryoprotectants which raises the glass transition temperature range of the sample. One or more dry protectants may be added to the cryosolution. The cryosolution is then nebulized and rapidly cooled with novel apparatus, dried by molecular distillation, stored and then rehydrated in a buffer prior to its use. The solutions comprise novel mixtures of cryoprotectants. <IMAGE></p> |
申请公布号 |
EP0475409(A3) |
申请公布日期 |
1993.09.01 |
申请号 |
EP19910115480 |
申请日期 |
1991.09.12 |
申请人 |
LIFECELL CORPORATION |
发明人 |
LIVESEY, STEPHEN;DEL CAMPO, ANTHONY A.;NAG, ABHIJIT;NICHOLS, KEN B.;PIUNNO, CARMEN;ROSS, DAVID |
分类号 |
G01N1/28;A01N1/02;A61K35/18;A61K39/13;C12N1/04;F25C1/14;F26B5/06;(IPC1-7):A01N1/02 |
主分类号 |
G01N1/28 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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