Assay for hepatitis a virus

摘要

A quantitative assay for HAV comprises infecting at a temperature of from about 30 DEG to about 37 DEG C. a cell sheet with a dilution series of hepatitis A virus and then adding Newcastle disease virus (NDV) to the infected cell sheet and incubating the infected cell sheet in the presence of NDV at an elevated temperature for a time sufficient to permit a cytopathic effect (CPE) to become manifest, this time typically being at least about 4 days and preferably about 5 days. At an incubation temperature of from about 31 DEG to about 33 DEG C. the absence of CPE indicates the presence of HAV and the presence of CPE indicates the absence of HAV. At an incubation temperature of from about 34 DEG to about 36 DEG C. the absence of CPE indicates the absence of HAV and the presence of CPE indicates the presence of HAV.