Method to determine a diagnostic indicator of blood sugar condition, and, a liquid chromatographic microcolumn therefor

摘要

A method to determine a numerical percentage value as a diagnostic indicator of the blood sugar condition of a specific person. A whole blood sample is lysed and introduced into an improved ion exchange cellulose (Whatman CM-52) liquid column for microchromatographic separation of hemolysates. Amounts of hemoglobin species, particularly Hb-A1a-c, are detected and measured by spectrometric (color) analysis. A mathematical computation using integer factors corresponding to amounts of various hemoglobin species produces the numerical percentage value.