Recombinant Cells and Organisms Having Persistent Nonstandard Amino Acid Dependence and Methods of Making Them

摘要

Novel recombinant cells and recombinant organisms persistently expressing nonstandard amino acids (NSAAs) are provided. Methods of making novel recombinant cells and recombinant organisms dependent on persistently expressing NSAAs for survival are also provided. These methods may be used to make safe recombinant cells and recombinant organisms and/or to provide a selective pressure to maintain one or more reassigned codon functions in recombinant cells and recombinant organisms.

主权项

1. A recombinant cell comprising:
An essential polypeptide encoded by an essential nucleic acid sequence, said essential polypeptide having a nonstandard amino acid substitution at a particular position, wherein the absence of the nonstandard amino acid substitution at the particular position disrupts one or any combination of translation, folding and function of the essential polypeptide, and wherein the recombinant cell dies or experiences reduced or no proliferation if one or more of translation, folding or function of the essential polypeptide is disrupted.