HIGH-THROUGHPUT DETECTION METHOD FOR SINGLE-TO-MULTIPLE NUCLEOTIDE MUTATIONS BASED ON NANOPARTICLE-DNA CORONA COMPLEX VIA KELVIN PROBE FORCE MICROSCOPY

摘要

The present invention relates to a high-throughput detection method for single to multiple nucleotide mutations based on a DNA-nanoparticle complex using kelvin probe force microscopy (KPFM) and, more specifically, to a method for identifying the mutation of genes by fixing probe DNA to nanoparticles, spreading a compound hybridized with target DNA on a substrate, and using the surface potential measured by the KPFM.