| 摘要 |
PURPOSE:In fermentation of mannitol with lactic acid bacteria, to produce mannitol in a short time in high yield and in high productivity, by adjusting pH of a culture solution to a specific value. CONSTITUTION:Lactic acid bacteria such as Lactobacillus brevis, etc., capable of producing mannitol is inoculated into a liquid medium which has >=10% saccharide concentration and preferably contain betaine. Then, pH of the culture solution is kept at 5.0-6.0 from beginning of culture. When the pH of the culture solution becomes <=5, an alkali is added to the culture solution to make the pH >=5. When the pH of the culture solution becomes >=6.0, an acid is added to the culture solution to make the pH <=6.0. Culture is carried out while regulating the pH. |
