人多能干细胞用培养基质及其用途,申请号CN201410776254.2-传众专利搜索

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发明名称	人多能干细胞用培养基质及其用途
摘要	本发明提供能够在无饲养细胞的培养环境中使人多能干细胞在保持多能性的状态下进行维持培养的培养基质以及使用该培养基质的人多能干细胞的培养方法。在以优选0.5μg/cm2～25μg/cm2的浓度涂布有人层粘连蛋白α5β1γ1的E8片段或人层粘连蛋白α3β3γ2的E8片段的人多能干细胞培养用培养基质上，以4×10<sup>4</sup>细胞/cm<sup>2</sup>～10×10<sup>4</sup>细胞/cm<sup>2</sup>的密度对分散为单个细胞的人多能干细胞进行接种，由此使人多能干细胞在保持多能性的状态下快速扩增。
申请公布号	CN104498430A	申请公布日期	2015.04.08
申请号	CN201410776254.2	申请日期	2010.10.07
申请人	国立大学法人大阪大学;国立大学法人京都大学	发明人	关口清俊;二木杉子;溪口征雅;林麻利亚;中辻宪夫;宫崎隆道;川濑荣八郎;末盛博文
分类号	C12N5/071(2010.01)I;C12N5/074(2010.01)I;C12N5/10(2006.01)I	主分类号	C12N5/071(2010.01)I
代理机构	中原信达知识产权代理有限责任公司 11219	代理人	于洁;王海川
主权项	一种人ES细胞以外的人多能干细胞培养用培养基质，其特征在于，涂布有人层粘连蛋白α5β1γ1的E8片段或人层粘连蛋白α3β3γ2的E8片段。
地址	日本大阪府

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