发明名称 |
TWO-WAY, PORTABLE RIBOSWITCH MEDIATED GENE EXPRESSION CONTROL DEVICE |
摘要 |
A regulatable gene expression construct comprising a nucleic acid molecule comprising a two-way riboswitch operably linked to a target sequence. Also provided is a library screening strategy for efficient creation of target-specific riboswitches. A theophylline-repressible and IPTG-inducible riboswitch device achieves portable control of gene expression control in a ‘two-way’ manner. The default state of target genes is ON; the targets are switched off by adding theophylline, and switched back to the ON-state by adding IPTG without changing growth medium. The riboswitch device regulates gene expression in a portable, adjustable, and two-way manner with a variety of scientific and biotechnological applications. |
申请公布号 |
EP2726608(A4) |
申请公布日期 |
2015.03.11 |
申请号 |
EP20120805000 |
申请日期 |
2012.06.29 |
申请人 |
THE UNIVERSITY OF HONG KONG |
发明人 |
HUANG, JIANDONG;JIN, YE |
分类号 |
C12N15/09;C12N15/11;C12N15/113;C12N15/115;C12N15/56;C12N15/63;C12N15/67;C12N15/72;C12N15/90 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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