| 摘要 |
A method for increasing drought tolerance in a plant or photosynthetic organism is disclosed, where the tolerance is increased by introducing nucleic acid sequences coding for polypeptides with monooxigenase activity or by the modification of endogenous nucleic acid sequences that increase the endogenous TMAO content in plants as well as to nucleic acid constructs, recombinant vectors, cells, transgenic plants, crops, propagation material, compositions and harvestable parts of a plant comprising nucleic acid sequences in order to increase water stress tolerance and so on. |
| 主权项 |
1. A method of producing a transgenic drought tolerant plant, plant cell, or plant tissue, wherein the method comprises transforming a plant, plant cell, or a plant tissue with a nucleic acid sequence encoding at least eight copies of FMO protein coding sequence SEQ ID NO: 2 operably linked to a promoter, and selecting for a transgenic plant, plant cell, or plant tissue, wherein expression of the at least eight copies of the FMO protein coding sequence in said transgenic plant, plant cell, or plant tissue induces an increased expression of trimethylamine N-oxide at least three times greater than the expression of trimethylamine N-oxide in a non-transgenic plant, plant cell, or plant tissue, and wherein said increased expression of trimethylamine N-oxide in said transgenic plant, plant cell, or plant tissue catalyzes the oxidation of endogenous metabolites containing nucleophilic nitrogen. |
