AGGREGATES OF SUPEROXIDE DISMUTASE

摘要

The present invention provides a method for typing an aggregate (including a fibril) of a polypeptide in a test sample, such as human superoxide dismutase (hSODI), wherein the polypeptide is capable of forming at least two structurally distinct types of aggregates, said method comprising (i) determining the reactivity of the aggregate in the test sample with one or more different antibody preparations in a panel of antibody preparations, wherein each antibody preparation in the panel has binding specificity to a peptide sequence derived from the amino acid sequence of the polypeptide, such that different antibody preparations in the panel have binding specificity to different peptides, and wherein at least one of the antibody preparations of the panel, for which reactivity to the aggregate is determined in step (i), displays differential reactivity to at least two structurally distinct types of the aggregate; and (ii) attributing a type to the aggregate in the test sample, based on the determined level(s) of reactivity with the one or more antibody preparations in the panel as determined in step (i).