Methods of inducing regulated pancreatic hormone production in non-pancreatic islet tissues

摘要

Disclosed are methods and pharmaceutical compositions for inducing pancreatic hormone production.

主权项

1. A method of inducing endogenous PDX-1 expression in a human differentiated primary non-pancreatic cell, said method comprising:
(i) introducing to said human differentiated primary non-pancreatic cell a composition comprising a nucleic acid sequence operably linked to a promoter encoding an exogenous PDX-1 polypeptide, and (ii) expressing the PDX-1 polypeptide in the cell of step (i), wherein expression of said exogenous PDX-1 polypeptide induces expression of endogenous PDX-1 and said human differentiated primary non-pancreatic cell is a bone marrow, muscle, spleen, kidney or liver cell.