Synthesis of 2',3'-dideoxynucleosides for automated DNA synthesis and pyrophosphorolysis activated polymerization

摘要

Methods for preparation of 2',3'-dideoxynucleotides support structures, such as 2',3'-dideoxyguanosine, 2',3'-dideoxyadenosine, and 3'-deoxythymidine support structures are disclosed. Various methods of using such structures are also provided, such as their use for automated DNA synthesis and pyrophosphorolysis activated polymerization.