发明名称 |
LIGAND SCREENING AND DISCOVERY |
摘要 |
Disclosed is a method that includes: (i) providing a plurality of initial nucleic acid cassettes that include: a) a first coding region encoding a first immunoglobulin variable domain, b) a second coding region encoding a second immunoglobulin variable domain, and c) a ribosomal binding site disposed between the first and second coding regions for translation of the second polypeptide in a first expression system, wherein the first and second coding regions are in the same translational orientation; (ii) modifying each nucleic acid cassette of the plurality in a single reaction mixture so that it is functional in a second expression system, wherein the first and second region remain physically attached during the modifying; (iii) introducing each modified nucleic acid cassette into a mammalian cell to produce a mixture of transfected cells; and (iv) expressing each modified nucleic acid cassette in the transfected cells.
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申请公布号 |
US2013274112(A1) |
申请公布日期 |
2013.10.17 |
申请号 |
US201313867806 |
申请日期 |
2013.04.22 |
申请人 |
HOOGENBOOM HENRICUS R J.;MULLBERG JURGEN;LADNER ROBERT C.;DYAX CORP. |
发明人 |
HOOGENBOOM HENRICUS R J.;MULLBERG JURGEN;LADNER ROBERT C. |
分类号 |
C12N15/10;C07K16/00;C12N15/85 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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