发明名称 |
PRODUCTION OF ACETYL-COENZYME A DERIVED ISOPRENOIDS |
摘要 |
Provided herein are compositions and methods for the heterologous production of acetyl-CoA-derived isoprenoids in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an acetaldehyde dehydrogenase, acetylating (ADA, E.C. 1.2.1.10) and an MEV pathway comprising an NADH-using HMG-CoA reductase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an ADA and an MEV pathway comprising an acetoacetyl-CoA synthase. In some embodiments, the genetically modified host cell further comprises one or more heterologous nucleotide sequences encoding a phosphoketolase and a phosphotransacetylase. In some embodiments, the genetically modified host cell further comprises a functional disruption of the native PDH-bypass. The compositions and methods described herein provide an energy-efficient yet redox balanced route for the heterologous production of acetyl-CoA-derived isoprenoids. |
申请公布号 |
CA2853679(A1) |
申请公布日期 |
2013.05.16 |
申请号 |
CA20122853679 |
申请日期 |
2012.11.09 |
申请人 |
AMYRIS, INC. |
发明人 |
GARDNER, TIMOTHY STEVENS;HAWKINS, KRISTY MICHELLE;MEADOWS, ADAM LEON;TSONG, ANNIE ENING;TSEGAYE, YOSEPH |
分类号 |
C12P23/00;C12N15/52;C12P5/00 |
主分类号 |
C12P23/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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