GENE-BASED ADJUVANTS AND COMPOSITIONS THEREOF TO INCREASE ANTIBODY PRODUCTION IN RESPONSE TO GENE-BASED VACCINES

摘要

DNA vectors encoding IL-12, IL-21, and various soluble trimeric TNFSF ligands to increase antibody titers for DNA vaccines are disclosed herein. The DNA vectors include IL-12, IL-21, and TNFSF ligands, either individually or in particular combinations, and increase antibody production to the gpl40 protein of HIV- 1 and increase virus neutralizing antibodies to HIV-1. Compositions, kits, and methods comprising DNA vectors encoding IL-12, IL-21, and various soluble trimeric TNFSF ligands may be utilized in treating disorders or diseases and conditions such as HIV infection and cancer.