Sensor chip with receptors included in a polymeric network

摘要

The biosensor chip for detecting ligands, has carrier (2) whose surface (3) is provided with two test sites (4a, 4b) at which two receptors which bind specifically to a ligand are immobilized, and a swellable polymer network (8) covalently fixed to the second test site. At a first test site, first receptors are covalently bonded to the surface in a monolayer and/or in several molecular layers, which are layered on top of one another. The second receptors, which bind specifically to the ligand, are immobilized on and/or in the polymer network. The biosensor chip for detecting ligands, has carrier (2) whose surface (3) is provided with two test sites (4a, 4b) at which two receptors which bind specifically to a ligand are immobilized, and a swellable polymer network (8) covalently fixed to the second test site. At a first test site, first receptors are covalently bonded to the surface in a monolayer and/or in several molecular layers, which are layered on top of one another. The second receptors, which bind specifically to the ligand, are immobilized on and/or in the polymer network. Into the surface, optical detectors (5a, 5b) for detecting the binding of the ligands at the receptors, is integrated, which form a unit with the carrier, and the polymer network is fixed directly or indirectly at the detectors over a site of the receptors. The polymer network exhibits hydrophilic copolymers and contains water-soluble, preformed polymer, which exhibits a photo-linkable benzophenone group, and a polymer, which exhibits phosphonic acid group as an adhesive agent. Both receptors exhibit same and different molecules. The first receptor is immobilized at the surface over a group of silanes. The carrier consists of transparent plastic and exhibits a semiconductor material. The polymer network is arranged on the first receptors and is fixed indirectly at the surface of the carrier. The detector is integrated into the semiconductor material, and the polymer network containing the receptors is directly arranged on the surface of the detector. The detectors detect the binding of the ligands at the receptors dependent upon the luminescence radiation and measure the binding of the ligands at the receptors dependent upon the electric field and/or an electric characteristic of the ligands. Sensor arrangements are intended at the carrier. The carrier is a flexible polymer strip foil, and the sensor arrangements are arranged to each other in extending direction of the strip foil. The sensor chip forms a limiting wall of a flow-measuring chamber, which is formed with inlet- and outlet opening. The measuring chamber exhibits two compartments to which a sensor arrangement arranged in each case at the surface of the carrier adjoins. An independent claim is included for a procedure for the measurement of a concentration of a ligand contained in an analyte solution.