Bisulfite conversion of DNA, involves reacting genomic DNA with bisulfite reagent, in presence of compound out of group of dioxane, one of its derivatives and similar aliphatic cyclic ether

摘要

Bisulfite conversion (M1) of DNA, involves reacting genomic DNA with a bisulfite reagent, where the reaction is carried out in the presence of a compound out of the group of dioxane, one of its derivatives and a similar aliphatic cyclic ether. Bisulfite conversion (M1) of DNA, involves reacting genomic DNA with a bisulfite reagent, where the reaction is carried out in the presence of (a) a compound out of the group of dioxane, one of its derivatives and a similar aliphatic cyclic ether, (b) a compound of formula called (1), or (c) the reaction is conducted at a temperature in the range of 0-80 [deg]C and that the reaction temperature is increased to a range of 85-100 [deg]C briefly during the course of the conversion (thermospike). [Image] n : 1-35000; m : 1-3;and R 1,R 2H, Me, Et, Pr or Bu. An independent claim is also included for a kit, comprising a reagent containing bisulfite, denaturing reagents or solvents, and scavengers and primers for the production of amplificates, and optionally an ultrafiltration tube or instructions for performing (M1).