发明名称 |
Method for generating diversity |
摘要 |
The invention relates to a method for preparing an antibody-producing cell line capable of directed constitutive hypermutation of a specific nucleic acid region, comprising the steps of: a) screening a clonal cell population for V gene diversity; b) isolating one or more cells which display V gene diversity and comparing the rate of accumulation of mutations in the V genes and other genes of the selected cells; and c) selecting a cell in which the rate of V gene mutation exceeds that of other gene mutation.
|
申请公布号 |
US2005026246(A1) |
申请公布日期 |
2005.02.03 |
申请号 |
US20030733532 |
申请日期 |
2003.12.11 |
申请人 |
MEDICAL RESEARCH COUNCIL |
发明人 |
SALE JULIAN EDWARD;NEUBERGER MICHAEL SAMUEL;CUMBERS SARAH JANE |
分类号 |
C12N15/00;A01K67/027;C07K16/00;C12N5/08;C12N5/10;C12N9/00;C12N15/09;C12N15/10;C12P21/02;(IPC1-7):C07K16/18;C07H21/04;C12N5/06 |
主分类号 |
C12N15/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|