METHOD OF USING ADENOVIRAL VECTORS WITH INCREASED PERSISTENCEIN VIVO

摘要

The invention provides a method of expressing an exogenous nucleic acid in a mammal. The method comprises slowly releasing into the bloodstream a dose of replication-deficient or conditionally-replicating adenoviral vector having reduced ability to transduce mesothelial cells and hepatocytes. The normaliz ed average bloodstream concentration of the adenovirus over 24 hours post- administration is at least about 1%. Alternatively, the normalized average bloodstream concentration over 24 hours post-administration is at least abou t 5-fold greater than the normalized average bloodstream concentration for an equivalent dose of a wild-type adenoviral vector. A method of destroying tum or cells in a mammal also is provided.