METHODS FOR PREPARING ANTIGEN MASKED RED BLOOD CELLS HAVING REDUCED HEMOLYSIS BY SERA

摘要

Methods are provided for the preparation of an RBC composition having significantly reduced antigenicity and having reduced levels of hemolysis by any serum or plasma sample. The methods of preparation of the red cell compositions involve the reaction of an activated antigen masking compound having a molecular weight of approximately 20-40 kDa, wherein the resulting red cells are not readily hemolyzed by any serum or plasma sample, for example by complement lysis. The RBC compositions are of particular use for introduction into an individual in cases where the potential for an immune reaction is high, for example in alloimmunized blood recipients or in trauma situations where the possibility of transfusion of a mismatched unit of blood is higher.