IMMORTALIZED DENDRITIC CELL LINE ORIGINATING IN BONE MARROW

摘要

It is intended to provide an immortalized dendritic cell line sustaining the functions and properties inherent to dendritic cells, a method of establishing the same, a method of screening a useful substance by using the above immortalized dendritic cell line, and a cell vaccine comprising the above immortalized dendritic cell line as the main component. Namely, an immortalized dendritic cell line having an ability to take up an antigen, an ability to present an antigen and an ability to introduce CTL activity is established by hemolyzing bone marrow cells of a transgenic mouse carrying a large T antigen gene of a temperature-sensitive SV40 mutant strain tsA58 transferred thereinto, removing lymphocytes and Ia-positive cells therefrom, culturing the thus obtained cells in the presence of GM-CSF to induce dendritic cells, repeatedly subculturing the cells 10 times or more, and thus expressing myeloid molecules and leukocyte molecules on the cell surface.