发明名称 |
SELF-REPLICATING RNA MOLECULE FROM HEPATITIS C VIRUS |
摘要 |
A unique HCV RNA molecule is provided having an enhanced efficiency of establishing cell culture replication. Novel adaptive mutations have been identified within the HCV non-structural region that improves the efficiency of establishing persistently replicating HCV RNA in cell culture. This self-replicating polynucleotide molecule contains, contrary to all previous reports, a 5'-NTR that can be either an A as an alternative to the G already disclosed and therefore provides an alternative to existing systems comprising a self-replicating HCV RNA molecule. The G-->A mutation gives rise to HCV RNA molecules that, in conjunction with mutations in the HCV non-structural region, such as the G(2042)C/R mutations, possess greater efficiency of transduction and/or replication. These RNA molecules when transfected in a cell line are useful for evaluating potential inhibitors of HCV replication. |
申请公布号 |
WO02052015(A3) |
申请公布日期 |
2003.11.20 |
申请号 |
WO2001CA01843 |
申请日期 |
2001.12.20 |
申请人 |
BOEHRINGER INGELHEIM (CANADA) LTD.;KUKOLJ, GEORGE;PAUSE, ARNIM |
发明人 |
KUKOLJ, GEORGE;PAUSE, ARNIM |
分类号 |
G01N33/50;C07K14/18;C12N5/02;C12N5/10;C12N7/01;C12N15/09;C12N15/40;C12N15/51;C12N15/86;C12Q1/02;C12Q1/68;G01N33/15 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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