Method for amplifying RNA

摘要

Poly(A)<+> RNA extracted from a cell or a tissue is amplified by the following steps of: (a) synthesizing a cDNA first strand by using RNA extracted from the cell or the tissue as a template and a first primer having an oligo-dT and a promoter sequence, (b) joining a sequence to which a second primer can anneal to the 3' end of the cDNA first strand, (c) synthesizing a cDNA second strand by using the cDNA first strand as a template and the second primer to obtain a double-stranded cDNA, and (d) synthesizing RNA by using the double-stranded cDNA obtained in the step (c) and an RNA polymerase corresponding to the promoter sequence.