摘要 |
<p>This invention provides a method for detection of multiple kinases or multiple kinase substrates, whereby the presence and phosphorylation state of a large number kinases and/or kinase substrate proteins may be tracked in a single sample electrophoretically separating in one dimension, proteins in a sample to be tested for kinase or kinase substrate content; to produce an array of proteins so separated contacting the array with two or more antibodies selected from anti-kinase and anti-kinase substrate antibodies; and, detecting the presence of said antibodies bound to kinases or kinase substrate moieties in the array. This method may also comprise recording one or more values representative of a location for each of said detected antibodies bound to proteins in the array indicative of a location of a kinase or kinase substrate in the array.</p> |