| 摘要 |
PROBLEM TO BE SOLVED: To obtain a new ABC transporter gene encoding a polypeptide constituting an ATP-binding cassette transporter of Pseudomonas fluorescens and capable of efficiently producing useful proteins, e.g. lipase, by microbes. SOLUTION: This new ABC transporter gene is such one as to encode a polypeptide constituting an ATP-binding cassette transporter of Pseudomonas fluorescens, consisting of e.g. a polypeptide having the amino acid sequence described in formula I to III, or a polypeptide having an amino acid sequence wherein one or several amino acid residue(s) is (are) added, deleted or substituted in the above amino acid sequence and to efficiently produce useful proteins, e.g. lipase through the function of secreting proteins extracellularly. This gene is obtained by separating a chromosomal DNA from the Pseudomonas fluorescens No.33 strain and conducting PCR using a primer consisting of a partial sequence of the ABC transporter gene. |
