| 摘要 |
PURPOSE:To obtain a D-selenocystine decomposition enzyme reacting specifically to D isomer of selenocystine, by culturing a bacterial strain belonging to Clostridium genus in a nutrient medium. CONSTITUTION:A bacterial strain of Clostridium genus such as Clostridium sticklandi ATCC 12662, Clostridium sporogenes ATCC 11437, ATCC 7955, etc., is cultured in a medium containing carbon source, nitrogen source, inorganic salts and optionally minor metal salts, vitamins, etc., at 10-45 deg.C (preferably 20-42 deg.C, especially 25-40 deg.C) for about 5-48hr under anaerobic condition and the objective decomposition enzyme is separated from the cultured product. The enzyme has high substrate specificity to D-selenocystine and catalyzes the reaction of formula. |
