| 摘要 |
<p>In the prodn. of human fostal cell cultures, (a) virus free human 3-6 month foetal material is cut into small pieces and washed with Hanks soln. pH 7.2; (b) the comminuted material is incubated with 25% trypsin soln. (changed every 15-30 mins.) at 37 degrees C and pH 7.5 for 20-30 mins.; (c) suspended cells are syphoned off and this procedure repeated until all cells are in suspension; (d) the resulting cells are incubated ca 2 days at 37 degrees C without agitation and dead cells are removed; (e) the cultures are incubated 3-4 days without agitation, the nutrient medium being changed every 2-4 days; (f) optionally, cells are separated from the glass walls by incubating 15-30 mins. with 0.2% trypsin soln. at 37 degrees C, and then subjected to a second incubation as in step (e); (g) when only 30% of the cells are still mitotic, the culture is centrifuged; and (h) the resulting cells are suspended in 15% glycerine-contg. phosphate buffer at a concn. of ca 2000 cells/3 mm3. Administration i.v. or i.m. in the treatment of heart disease, kidney disease, liver disease, chronic degenerative disease, circulatory disorders, genital disease and nervous disorders, and to counteract the effects of ageing. The foetal cells used must be in an indifferent state. They can be used instead of fresh animal cells in the cell therapy method of Niehans.</p> |
