发明名称 |
Immobilized proteins and methods and uses thereof |
摘要 |
The invention relates to the field of covalently attaching proteins to a substrate, particularly to methods of immobilizing proteins by posttranslationally modifying a cysteine residue of said protein through the addition of functional groups. The invention also relates to biological molecules used in such techniques, including proteins, and detection methods and kits that utilize such immobilized proteins, such as a microdevice or “protein chip”, a high-throughput screening device, and for the microscopy of proteins on a surface. |
申请公布号 |
US9290581(B2) |
申请公布日期 |
2016.03.22 |
申请号 |
US201314139440 |
申请日期 |
2013.12.23 |
申请人 |
University of Utah Research Foundation |
发明人 |
Poulter Charles Dale;Labadi Guillermo Roberto;Gauchet Cecile;Hawkins Bohaty Rochelle Frances |
分类号 |
C07K17/14;C07K1/107;C07K1/113;C07K14/435;C12N9/10;G01N33/543;G01N33/552;C07K14/00;C12P21/00 |
主分类号 |
C07K17/14 |
代理机构 |
Thorpe North & Western, LLP |
代理人 |
Thorpe North & Western, LLP |
主权项 |
1. A method of covalently immobilizing a protein, comprising:
a. posttranslationally modifying a cysteine residue of said protein through the addition of functional groups, and b. immobilizing said protein by ligation of said functional groups to a substrate; wherein said protein comprises a soluble protein or a solubilized protein, and wherein said substrate comprises one or more of an azide-derivatized surface and an alkyne-derivatized surface. |
地址 |
Salt Lake City UT US |