QUANTIFICATION OF LIPOPROTEINS

摘要

Some aspects of this disclosure provide methods and reagents for the detection of apolipoproteins in a sample, for example, a sample obtained from a human subject. Some aspects of this disclosure provide protocols and reagents for the quantification of lipoproteins and lipoprotein particle populations comprising specific combinations of non-integral apolipoproteins and integral apolipoproteins, which is useful, inter alia, for the detection of diseases such as cardiovascular disease as well as an assessment of the risk of an individual to develop a disease.

主权项

1. A method of detecting an apolipoprotein in a sample, the method comprising
(a) contacting a sample comprising a lipoprotein particle with a binding agent that specifically binds a non-integral apolipoprotein, thereby depleting the sample of the non-integral apolipoprotein; (b) contacting the sample depleted of the non-integral apolipoprotein with a binding agent that specifically binds an integral apolipoprotein; (c) detecting an integral apolipoprotein in or on a lipoprotein particle bound to the binding agent of (a) and/or detecting an integral apolipoprotein in or on a lipoprotein particle bound to the binding agent of (b).