| 摘要 |
Disclosed is a method and kit of detecting a target material using an aptamer, and more particularly to a method and kit for detecting a target material, in which a sample and a second aptamer are added to a first aptamer immobilized on a solid phase so as to form a bond sandwiched between the first aptamer, the target material and the second aptamer, to an FET sensor-based method and kit for detecting a target material, and to an AAO sensor-based method and kit for detecting a target material.;The method for a target material using an aptamer can detect even low-molecular-weight materials which were difficult to detect in the prior art, thereby enabling detection of disease-related metabolites, environmental pollutants and food toxins in solutions. In addition, the detection method is a direct and simple method and is highly cost-effective, because it uses the aptamer which can be consistently reproduced and can be produced at low costs. |
| 主权项 |
1. A field effect transistor (FET) sensor-based method of detecting a small, apolar molecule using aptamers, the method comprising the steps of:
(a) adding a sample containing a small, apolar molecule, and a second aptamer binding specifically to the small, apolar molecule, to an FET sensor comprising a substrate, a source metal electrode and a drain metal electrode, which are formed on both sides of the substrate, respectively, and a gate formed on the substrate so as to come into contact with the source and drain metal electrodes, wherein a first aptamer binding specifically to the small, apolar molecule is immobilized as a probe on any one or more of the surfaces of the source metal electrode, the gate and the drain metal electrode; and (b) measuring a change in electric current between the source metal electrode and drain metal electrode of the FET sensor, the change occurring when the small, apolar molecule and the second aptamer bind to the first aptamer immobilized on the FET sensor, thereby detecting the small, apolar molecule. |
