| 摘要 |
<p>Disclosed is a disposable cartridge (2) that comprises a body (47), a bottom layer (3) with a first hydrophobic surface (17'), a top layer (4) with a second hydrophobic surface (17"), and a gap (6) between. The bottom layer (3) is as a flexible film that is sealingly attached its circumference (40) to the top layer (4). The disposable cartridge (2) is devoid of a spacer (5) that is located between the flexible bottom layer (3) and the top layer (4). The top layer (4) comprises loading sites (41) for transferring processing liquids, reagents or samples into the gap (6). When using this disposable cartridge (2), the bottom layer (3) that is configured as a working film for manipulating samples in liquid droplets (23) thereon is placed on an electrode array (9) of a digital microfluidics system (1), which comprises a base unit (7) with a cartridge accommodation site (8), an electrode array (9) located at said cartridge accommodation site (8). The electrode array (9) is supported by a bottom substrate (11), substantially extends in a first plane and comprises a number of individual electrodes (10). The digital microfluidics system (1) also comprises a central control unit (14) for controlling the selection of the individual electrodes (10) of said electrode array (9) and for providing these electrodes (10) with individual voltage pulses for manipulating liquid droplets within the gap (6) of said cartridge (2) by electrowetting. Also disclosed are a selection of disposable cartridges (2) and a method for manipulating samples in liquid droplets (23) that adhere to a hydrophobic surface (17).</p> |
