| 摘要 |
The present invention relates to methods and apparatus for conducting nucleic acid sequencing. The method comprises the steps of providing a platform having at least one well for containing at least one support surface, and providing at least one support 5 surface within each well, wherein the support surface is adapted to immobilise a first binding partner or selectively immobilise a second binding partner. The method further comprises the steps of binding or immobilising the first or second binding partner to the support surface and dispensing into each well from a point external of said platform a reagent, wherein after the dispensing step the platform is rotated sufficiently such that 10 any residual or unreacted said reagent is substantially centrifugally removed from each well and/or each support surface, wherein during rotation each support surface is held within each well. The invention also. relates to kits and uses of the kits for conducting nucleic acid sequencing. In particular, the invention has been developed primarily for use in sequencing nucleic acid by pyrosequencing, however the invention is not limited 15 to this field. V CD{00 I's) |
