COMPOSITION FOR SIMULTANEOUSLY DETECTING MYCOBACTERIUM TUBERCULOSIS AND NONTUBERCULOUS MYCOBACTERIA BY MEANS OF REAL-TIME MULTIPLEX POLYMERASE CHAIN REACTION COMPRISING NESTED HYBRIDIZATION PNA PROBE SYSTEM HAVING PARALLEL BINDING STRUCTURE, AND METH

摘要

The present invention relates to a composition for simultaneously detecting Mycobacterium tuberculosis and nontuberculous mycobacteria based on a nested hybridization peptide nucleic acid (PNA) probe system having a parallel binding structure, and to a method for simultaneously detecting Mycobacterium tuberculosis and nontuberculous mycobacteria using the composition and real-time polymerase chain reaction. Also, the present invention relates to a primer, a probe composition, and a method for analyzing to simultaneously detect Mycobacterium tuberculosis and nontuberculous mycobacteria based on the nested hybridization PNA probe system having the parallel binding structure. When carrying out the real-time multiplexed polymerase chain reaction by using the composition comprising the nested hybridized PNA probe system having the parallel binding structure, according to the present invention, Mycobacterium tuberculosis and nontuberculous mycobacteria can be simultaneously and accurately detected in a single tube with high sensitivity, thereby providing the advantage of making a more rapid and accurate clinical diagnosis.