发明名称 |
OPTIMIZATION OF CELLS FOR ENDOGENOUS GENE ACTIVATION |
摘要 |
The invention concerns a process for optimizing the gene expression in cells. A first aspect concerns a process for changing the expression of a nucleic acid sequence which is present endogenously in a eukaryotic cell by introduction of a heterologous expression control sequence into the genome of the cell by means of homologous recombination as well as site-specific recombinase-mediated excision of inserted foreign DNA and its replacement by further heterologous expression control sequences or/and amplification genes. In addition the invention concerns the introduction of one or several nucleic acid sequences to which an activator protein or an activator protein complex binds e.g. a hypoxia-inducible factor (HIF), into the genome of a eukaryotic cell by homologous recombination in order to change the expression of a target gene. Furthermore the invention concerns a process for testing the influence of 5' or 3' non-coding nucleic acid fragments on the expression of a target gene by determining the expression of a reporter gene. In addition the invention concerns a process for providing a DHFR-negative eukaryotic cell containing a recombinase target sequence as well as the expression of a nucleic acid sequence inserted into the recombinase target sequence.
|
申请公布号 |
CA2252970(C) |
申请公布日期 |
2011.11.15 |
申请号 |
CA19982252970 |
申请日期 |
1998.11.30 |
申请人 |
BOEHRINGER MANNHEIM GMBH |
发明人 |
HONOLD, KONRAD;HOLTSCHKE, THOMAS;STERN, ANNE |
分类号 |
C12N15/09;C12N15/90;C07K;C07K14/505;C12N;C12N5/00;C12N5/10;C12N5/22;C12N15/12;C12N15/63;C12N15/65;C12N15/67;C12N15/79;C12N15/85;C12Q;C12Q1/68;C12R1/91 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|