发明名称 |
Method for screening crystallization conditions in solution crystal growth |
摘要 |
A method of screening protein crystal growth conditions with picogram to microgram amounts of protein in picoliter or nanoliter volumes is provided. A preferred method comprises a microarray with a plurality of microchambers in the microarray. A protein solution is placed into the micro-chambers by an automated dispensing mechanism. The protein crystal growth conditions of each of the micro-chambers is adjusted so that the protein crystal growth conditions in at least two of the micro-chambers differs. Crystallization of the protein solution in the micro-chambers is effected. For example, crystallization can be effected by a precipitate solution and/or placing an oil barrier over the protein solution. Protein crystal growth in the micro-chambers is then observed.
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申请公布号 |
US7700363(B2) |
申请公布日期 |
2010.04.20 |
申请号 |
US20060639211 |
申请日期 |
2006.12.15 |
申请人 |
UAB RESEARCH FOUNDATION |
发明人 |
DELUCAS LAWRENCE J.;BRAY TERRY L. |
分类号 |
G01N33/00;B01J19/00;B01L3/00;B01L3/06;C30B7/00;C40B40/10;C40B50/08;C40B60/14;G01N25/14 |
主分类号 |
G01N33/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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