发明名称 |
HIGH-AFFINITY CHOLINE TRANSPORTER |
摘要 |
The present invention provides a protein having high- affinity choline transporter activity which is important physiologically, a gene encoding the protein, and a method of screening a material promoting the high-affinity choline transporter activity with the use of the same, and the like. By examining high-affinity choline uptake activity of Na+- dependent transporter cDNA deduced from the genomic sequence of a nematode (C. elegans) in a Xenopus oocyte expression system, the cDNA (cho-1) of nematode high-affinity choline transporter is identified. Then the cDNA (CHT1) of rat high-affinity choline transporter is cloned from rat spinal cord by using the homology of a base sequence to this cDNA as an index. Similarly, the cDNA of human high-affinity choline transporter is cloned from human genome.
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申请公布号 |
CA2382464(C) |
申请公布日期 |
2010.03.09 |
申请号 |
CA20002382464 |
申请日期 |
2000.08.18 |
申请人 |
JAPAN SCIENCE AND TECHNOLOGY CORPORATION |
发明人 |
HAGA, TATSUYA;OKUDA, TAKASHI |
分类号 |
C12N15/09;C12N15/12;A01K67/027;A61K38/00;A61K38/17;A61K45/00;A61K48/00;A61P25/28;C07K14/435;C07K14/47;C07K14/705;C07K16/18;C07K19/00;C12N5/10;C12P21/02;C12P21/08;C12Q1/00;C12Q1/68;G01N33/50;G01N33/53;G01N33/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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