MODIFIED BETA-LACTAMASE AND METHOD FOR ITS PREPARATION

摘要

The invention relates to targeted post translational modifi-cation of metallo-beta-lactamase by truncation and inser-tion of a dipeptide at the amino terminal end to reduce amino terminal heterogeneity in a recombinant DNA pro-duction system. A protein K-T-E-DeltaBL is expressed, and modified by host proteases to E-DeltaBL. Appropriate nucleotide molecules, vectors and hosts are also de-scribed. E-DeltaBL is useful in a pharmaceutical composition for treating antibiotic induced adverse effects in the intes-tine of patients treated with beta-lactam antibiotics.