Quantitative detection of biological contaminants in complex cell mixtures, useful for testing cultures in food processing, by flow cytometry, using fluorescent antibody and magnetic particles

摘要

Quantitative detection of biological contaminants (I) in complex cell mixtures, is new. Quantitative detection of biological contaminants (I) in complex cell mixtures comprises: (a) treating a sample of the cell mixture with a first antibody (Ab1), directed against (I) and labeled with a fluorescent dye, so that Ab1 binds to (I); (b) treating the sample with a second antibody (Ab2), directed against either (I) or Ab1 and coupled to a magnetic particle (MP), so that Ab2 binds; (c) applying a magnetic field; (d) transferring MP to a flow cytometer; and (e) detecting the fluorescent MP, bonded to (I) through Ab2.