Method to separate cells that have been modified with superparamagnetic particles by ballistic transfer

摘要

The ballistic transfer transfection technology employs a cold gas shock wave to accelerate microprojectiles that carry matter into cells by mechanical force. The present invention relates to a method that enables separation of transfected cells by co-adsorbing polynucleic acids and nanometer-sized superparamagnetic particles onto the microprojectiles. The transfected cells are rendered magnetically susceptible and can thus be separated by retaining them in a strong magnetic field. The use of the technology in a clinical context is facilitated.